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Anti- Ras(G13D) Mouse Monoclonal Antibody

价格¥4800.00
品牌NewEast Biosciences   
产地中国 武汉
货号26038
规格100ul
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  • Ras(G13D) [26038]
    [100 µL]

     

    Catalog Number:  26038
    Gene Symbol:  Ras(G13D)
    Description:   Anti Ras(G13D) Mouse Monoclonal Antibody
    Background:   Ras(G13D) is one type of Ras missense mutations at the codon 13. Ras protein is a membrane-associated GTPase that regulates cell proliferation, differentiation, and survival. Ras G13D mutation results in decreased GTPase activity and constitutive signaling. This mutation is found in many types of human cancers.
    Immunogen:    A synthetic peptide from the internal region of Ras which includes the mutation of G13D, human origin.
    Applications:   ELISA, WB, IF, IHC
    Recommended Dilutions:   
    ELISA: 1:100-1:3000 
    WB: 1:200-1:1000 
    IF: 1:50-1:100 
    IHC: 1:50-1:100
    Concentration:   1 mg/ml
    Host:   mouse
    Format:   Liquid
    Clonality:   Monoclonal
    Isotype:   IgG2b
    Purity:   Purified from ascites
    Storage buffer:
    Preservative:  no
    Constituents:  PBS (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 50% glycerol
    Species Reactivity:  RasG13D antibody recognizes RasG13D proteins of vertebrates.
    Storage Conditions:  Store at -20°C. Avoid freeze / thaw cycles
     
    Western blot:  
    Western blot analysis of recombinant Ras WT and G13D proteins with anti Ras(G13D) monoclonal antibody. Equal amounts of purified Ras WT (lane 1, Cat # 10172) and G13D (lane 2, Cat # 10155) proteins were loaded and blotted with anti Ras(G13D) mouse monoclonal antibody (Cat # 26038) (top panel). Input control (bottom panel) was blotted with rabbit polyclonal anti Ras antibody (Cat # 21021).
    Immunofluorescence:  
    Immunofluorescence of cells expressing RAS proteins with Anti Ras(G13D) antibody. HEK293T cells were transfected with pCDNA3-GFP Ras(G13D) plasmid,pCDNA3-GFP Ras(WT)plasmid, pCDNA3-GFP Ras(G13A) plasmid,pCDNA3-GFP Ras(G13V) plasmid, pCDNA3-GFP Ras(G13C)plasmid, pCDNA3-GFP Ras(G12R)plasmid or pCDNA3-GFP Ras(G13S)plasmid, then fixed and stained with anti-Ras(G13D) monoclonal antibody (Cat. # 26038).

     

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    Publications:
    1.  DNA vaccine elicits an efficient antitumor response by targeting the mutant Kras in a transgenic mouse lung cancer model
        Gene Therapy 21, 888-896 (October 2014)
    2.  Are all RAS mutations the same? Coexisting KRAS and NRAS mutations in a caecal adenocarcinoma and contiguous tubulovillous adenoma
        J Clin Pathol. 2015 Aug;68(8):657-60. doi: 10.1136

     

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