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Anti- HRAS(G13A) Mouse Monoclonal Antibody

价格¥4800.00
品牌NewEastBiosciences   
产地中国 武汉
货号26191
规格100ul
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  • Ras(G13A) [26191]
    [100ul]

     

    Catalog Number:  26191
    Gene Symbol:  HRas; KRas; NRas
    Description:   Anti HRAS(G13A) Mouse Monoclonal Antibody
    Background:   Belongs to the small GTPase superfamily. Ras family binds GDP/GTP and possess intrinsic GTPase activity, alternate between an inactive form bound to GDP and an active form bound to GTP. Activated by a guanine nucleotide-exchange factor (GEF) and inactivated by a GTPase-activating protein (GAP).Located in cell membrane, lipid-anchor, cytoplasmic side and Golgi apparatus membrane.
    Immunogen:   A synthetic peptide from the internal region of HRas which includes the mutation of G13A, human origin.
    Applications:   ELISA, WB, IHC
    Recommended Dilutions:   
    ELISA: 1:1000-1:5000 
    WB: 1:500-1:1000 
    IHC: 1:50-1:100
    Concentration:   1 mg/ml
    Host:   Mouse
    Format:   Liquid
    Clonality:   Monoclonal
    Isotype:   
    Purity:   Purified from ascites
    Storage buffer:
    Preservative:  no
    Constituents:  PBS (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 50% glycerol
    Species Reactivity:  Recognizes G13A mutant, but not wild-type H-RAS of vertebrates.
    Storage Conditions:   Store at -20°C. Avoid freeze / thaw cycles
     
    Western blot:  
    Western blot analysis of recombinant HRAS(G13A) and wildtype proteins. Purified His-tagged HRAS(G13A) protein (lane 2) and corresponding wildtype protein (lane 1) were blotted with Anti HRAS(G13A) monoclonal antibody (Cat. #26191).
    Immunofluorescence:   
    Immunofluorescence of cells expressing Ras proteins with Anti HRAS(G13A) antibody. HEK293T cells were transfected with pCDNA3-GFP HRas(WT) plasmid (left column) or pCDNA3-GFP HRAS(G13A) plasmid (right column), then fixed and stained with Anti HRAS(G13A) monoclonal antibody (Cat. #26191).

     

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    Publications:
    1.  DNA vaccine elicits an efficient antitumor response by targeting the mutant Kras in a transgenic mouse lung cancer model
        Gene Therapy 21, 888-896 (October 2014)
    2.  Are all RAS mutations the same? Coexisting KRAS and NRAS mutations in a caecal adenocarcinoma and contiguous tubulovillous adenoma
        J Clin Pathol. 2015 Aug;68(8):657-60. doi: 10.1136

     

     

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