武汉纽斯特生物技术有限公司欢迎您！

手机店铺

neweastbio@126.com

logo
搜索

 | 027-87561033

产品中心

PRODUCT CENTER

Anti -Active RhoA Mouse Monoclonal Antibody

价格￥4800.00
品牌NewEast Biosciences   
产地中国 武汉
货号26904
规格30μl
说明书说明书下载
  • 产品描述
  • 产品实验图文
  • 通路和蛋白
  • 技术资料
  • 文献引用
扫一扫购买

  • Active RhoA GTP [26904]
    [30 µL]

     

     

    Catalog Number:  26904
    Gene Symbol:   RHOA
    Description:   Anti Active RhoA Mouse Monoclonal Antibody
    Background:   Small GTPases are a super-family of cellular signaling regulators. RhoA belongs to the Rho sub-family of GTPases. Rho proteins play critical roles in many actin cytoskeleton- dependent processes including platelet aggregation, cell motility, contraction, and cytokinesis. It regulates the formation of stress fibers and focal adhesions in fibroblasts and Ca 2+ sensitivity of smooth muscle contraction.
    Immunogen:   Recombinant full length protein of active RhoA
    Applications:   IP, IHC
    Recommended Dilutions:   1 µg for 1~2 mg total cellular proteins for IP; 
                                                       1:100 dilution for IHC
    Concentration:   1 mg/ml
    Host:   Mouse
    Format:   Liquid
    Clonality:  Monoclonal
    Isotype:   IgG2b
    Purity:   Purified from ascites
    Storage buffer:
    Preservative:  no
    Constituents:  PBS (without Mg 2+ and Ca 2+ ), pH 7.4, 150 mM NaCl, 50% glycerol
    Species Reactivity:  Anti-active RhoA antibody recognizes active RhoA from vertebrates.
    Storage Conditions:  Store at -20°C. Avoid freeze / thaw cycles
     
    Immunoprecipitation/Western Blot:  

    IP/WB analysis of active RhoA protein. Purified recombinant RhoA proteins wer
    e loaded with GDP (lane 1) or GTPγS (lane 2). These proteins were immunoprecipitated
    with anti active RhoA mouse monoclonal antibody (Cat. # 26904). After SDS/PAGE, the
    membrane filter was probed with anti RhoA mouse monoclonal antibody (Cat. # 26007).

     

     

     

    Related Products
    Catalog# Name Size Price
    80601 RhoA pulldown Activation Assay Kit  30 assays  ￥6800  
    26904 Anti -Active RhoA Mouse Monoclonal Antibody  30 μL  ￥4800
    26007 Anti -RhoA Mouse Monoclonal Antibody  100 μL  ￥2800
    21009 Anti- RhoA Rabbit Polyclonal Antibody  100 μL  ￥2600

     

     

     

     

     

  • 示例结果

     

    下图展示的是武汉纽斯特生物技术有限公司的 RhoA 活性试剂盒的典型结果。下面的数据仅供参考。 

     

     

     

     

    Rho 活性分析。小鼠胚胎成纤维细胞（MEF）用血小板衍生因子（PDGF）处理（Lane 2）和未处理（Lane 1）。细胞裂解物用特异性识别 RhoA 活性构象的鼠单克隆抗体(Cat. # 26904)孵育(上图)。亲和沉淀物用anti-RhoA pAb(Cat # 21009)进行免疫印迹实验。底部的图展示的是细胞裂解液的活性 RhoA 的 Western blot 实验结果。

     

  • Predicted MW.   22 kDa

    Observed MW.   22 kDa

    Uniprot ID.       P61586

手机商铺
资讯热线

18062798497

（微信同步）

工作日9:00-18:00

在线留言

友情链接：

Copyright©2019武汉纽斯特生物技术有限公司版权所有   备案号:鄂ICP备13003341号-2   公安备案号42018502003460

技术支持：优狐