Anti Active Cdc42 Mouse Monoclonal Antibody活性抗体

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Anti Active Cdc42 Mouse Monoclonal Antibody

价格	￥4800.00
品牌	NewEastBio
产地	美国
货号	26905
种属	Mouse
规格	30μl
说明书	说明书下载

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Active Cdc42 GTP [26905]
[30 µL]

Catalog Number: 26905

Gene Symbol: CDC42

Description: Anti Active Cdc42 Mouse Monoclonal Antibody

Background: Small GTPases are a super-family of cellular signaling regulators. Cdc42 belongs to the Rho sub-family of GTPases that regulate cell motility, cell division, and gene transcription. GTP binding increases the activity of Cdc42, and the hydrolysis of GTP to GDP renders it inactive. GTP hydrolysis is aided by GTPase activating proteins (GAPs), while exchange of GDP for GTP is facilitated by guanine nucleotide exchange factors (GEFs).

Immunogen: Recombinant full length protein of active Cdc42

Applications: IP, IHC

Recommended Dilutions: 1 µg for 1~2 mg total cellular proteins for IP;
1:100 dilution for IHC

Concentration: 1 mg/ml

Host: mouse

Format: Liquid

Clonality: Monoclonal

Isotype: IgG1

Purity: Purified from ascites

Storage buffer:

Preservative: no

Constituents: PBS (without Mg²⁺ and Ca²⁺), pH 7.4, 150 mM NaCl, 50% glycerol

Species Reactivity: Anti active Cdc42 antibody recognizes active Cdc42 from vertebrates.

Storage Conditions: Store at -20°C. Avoid freeze / thaw cycles

Download Datasheet:

IP-Western blot:

IP/WB analysis of active Cdc42 protein. Purified recombinant Cdc42 proteins were loaded with GDP (lane 1) or GTPγS (lane 2). These proteins were immunoprecipitated with anti active Cdc42 mouse monoclonal antibody (Cat. # 26905). After SDS/PAGE, the membrane filter was probed with anti Cdc42 mouse monoclonal antibody (Cat. # 26008).

Immunofluorescence:

Immunofluorescent labeling of Cdc42-GTP in MS1 mouse endothelial cells. The MS1 cells cultured on glass coverslips (Fisher) in DMEM containing 5% FBS were fixed with 4% paraformaldehyde for 10 minutes at RT (room temperature) and permeablized in PBSN (PBS+0.1%NP40) for 15 minutes at RT. The cells were stained with primary antibody (Cdc42 GTP) at 1:25 dilution in Invitrogen's CAS-BLOCK (00-8120) reagent at RT for 1 hour. After brief washing in PBSN (3x5min), a secondary Alexa555 conjugated goat anti mouse antibody (Invitrogen A21424) was applied in 1:200 in CAS-BLOCK at RT for 1 hour. The coverslips were then washed in PBSN (3x5min) and mounted using vectorsheld's hard set mounting medium (H-1500), and examined using a Zeiss inverted epifluorescent miscroscope.

Related Products
Catalog#	Name	Size	Price
80701	Cdc42 Activation Assay Kit	20 assays	￥6,800
26905	Active Cdc42 GTP Monoclonal Antibody	30 μL	￥4,800
26008	Anti Cdc42 Mouse Monoclonal Antibody	100 μL	￥2800
21010	Anti Cdc42 Rabbit Polyclonal Antibody	100 μL	￥2600

Publications:

1. Vibrio parahaemolyticus Effector Proteins Suppress Inflammasome Activation by Interfering with Host Autophagy Signaling
PLoS Pathog. 2013 Jan;9(1):e1003142

2. Interferon gamma induces actin polymerization, Rac1 activation and down regulates phagocytosis in human monocytic cells
Cytokine. 2012 Jan;57(1):158-68

3. Invadopodia and rolling-type motility are specific features of highly invasive p190bcr-abl leukemic cells
European Journal of Cell Biology Volume 91, Issues 11–12, November–December 2012, Pages 978–987

4. Epithelial junction formation requires confinement of Cdc42 activity by a novel SH3BP1 complex
J Cell Biol. 2012 Aug 20;198(4):677-93

5. Polarized migration of lymphatic endothelial cells is critically dependent on podoplanin regulation of Cdc42
Am J Physiol Lung Cell Mol Physiol. 2011 Jan;300(1):L32-42

6. The Aarskog-Scott Syndrome Protein Fgd1 Regulates Podosome Formation and Extracellular Matrix Remodeling in Transforming Growth Factor beta-Stimulated Aortic Endothelial Cells
Mol Cell Biol. 2011 Nov;31(22):4430-41

7. MMP-9 and CXCL8/IL-8 Are Potential Therapeutic Targets in Epidermolysis Bullosa Simplex
PLoS One. 2013 Jul 19;8(7):e70123

8. p21-Activated Kinase (PAK) Regulates Cytoskeletal Reorganization and Directional Migration in Human Neutrophils
PLoS One. 2013 Sep 3;8(9):e73063

9. The Cdc42 Guanine Nucleotide Exchange Factor FGD6 Coordinates Cell Polarity and Endosomal Membrane Recycling in Osteoclasts
J Biol Chem. 2014 Jun 27;289(26):18347-59

10. Cdc42 Inhibitor and Uses Thereof
United States Patent Application 20140194451

11. The α4 Nicotinic Receptor Promotes CD4+ T-Cell Proliferation and a Helper T-Cell Immune Response
Molecular Pharmacology January 2014 vol. 85 no. 1 50-61

具备高特异性，高亲和力高灵敏度，操作简单迅速，性能稳定，数据可靠

科研领域（非临床）

Effects of age-dependent changes in cell size on endothelial cell proliferation and senescence through YAP1 2019-09-15
发表时间：Sarkar, Rajabrata，Dickinson, Chris J.，Stanley, James C.

名称/货号：Aging (Albany NY) 2019 Sep 15; 11(17): 7051–7069

影响因子：

应用范围：

种属：
Wiskott–Aldrich syndrome protein (WASP) is a tumor suppressor in T cell lymphoma 2019-01-25
发表时间：enotti, Matteo Ambrogio, Chiara Cheong, Taek-Chin Pighi, Chiara Mota, Ines Cassel, Seth H. Compagno, Mara Wang, Qi Dall’Olio,

名称/货号：Nat Med. 2019 Jan; 25(1): 130–140

影响因子：

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种属：
High glucose levels promote the proliferation of breast cancer cells through GTPases 2017-09-04
发表时间：Yilin Hou 1 , Man Zhou 1 , Jing Xie 1 , Ping Chao 1 , Qiyu Feng 2 , Jun Wu 1

名称/货号：Breast Cancer (Dove Med Press) 2017; 9: 429–436

影响因子：

应用范围：

种属：
An FAK-YAP-mTOR Signaling Axis Regulates Stem Cell-Based Tissue Renewal in Mice 2017-07-06
发表时间：Jimmy Kuang-HsienHu1WeiDu12Samuel J.Shelton34Michael C.Oldham34C. MichaelDiPersio5Ophir D.Klein167

名称/货号：Cell Stem Cell. 2017 Jul 6; 21(1): 91–106.e6.

影响因子：

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种属：
GGPP-Mediated Protein Geranylgeranylation in Oocyte Is Essential for the Establishment of Oocyte-Granulosa Cell Communication and Primary-Secondary Follicle Transition in Mouse Ovary 2017-01-13
发表时间：Chen Jiang,Fan Diao，Yong-Juan Sang，Na Xu，Rui-Lou Zhu，Xiuxing Wang

名称/货号：PLoS Genet. 2017 Jan; 13(1): e1006535

影响因子：

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种属：
Nitric oxide increases the migratory activity of non-small cell lung cancer cells via AKT-mediated integrin αv and β1 upregulation 2016-12-04
发表时间：Saisongkorh V1,2, Maiuthed A1,2, Chanvorachote P3,4.

名称/货号：Cell Oncol (Dordr). 2016 Jul 4

影响因子：

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种属：
Heme oxygenase-1 protects spinal cord neurons from hydrogen peroxide-induced apoptosis via suppression of Cdc42/MLK3/MKK7/JNK3 signaling 2016-11-18
发表时间：Wang S1, Zhang T2, Yang Z3, Lin J4, Cai B5, Ke Q1, Lan W4, Shi J1, Wu S1, Lin W6.

名称/货号：Apoptosis. 2016 Nov 18

影响因子：

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种属：
Thiopurine prodrugs mediate immunosuppressive effects by interfering with Rac1 function 2016-05-03
发表时间：Xiangle Sun，Michael Wey，Jin-Young Shin，Hope Umutesi

名称/货号：Journal of Biological Chemistry (Impact Factor: 4.57). 05/2016; DOI: 10.1074/jbc.M115.694422

影响因子：

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种属：
Mutations of Cystic Fibrosis Transmembrane Conductance Regulator (CFTR) Gene Cause a Monocyte-selective Adhesion Deficiency 2015-12-22
发表时间：Claudio Sorio 1,2*, Alessio Montresor 1, Matteo Bolomini-Vittori 1,2,3, Sara Caldrer 1,2, Barbara Rossi 1, Silvia Dusi 1, Stefano Angiari 1, Jan E. Johansson 1,2, Marzia Vezzalini 1,2,

名称/货号：Am J Respir Crit Care Med. 2015 Dec 22.

影响因子：

应用范围：

种属：
Curcumin inhibits growth of human breast cancer cells through demethylation of DLC1 promoter 2015-10-10
发表时间：Liu Y1,2, Zhou J3, Hu Y4,5, Wang J6, Yuan C7.

名称/货号：Mol Cell Biochem. 2016 Nov 10

影响因子：

应用范围：

种属：
Actin Remodeling by Nck Regulates Endothelial Lumen Formation 2014-12-08
发表时间：Chaki SP1, Barhoumi R2, Rivera GM3.

名称/货号：Mol Biol Cell. 2015 Jul 8. pii: mbc.E15-06-0338.

影响因子：

应用范围：

种属：
Comparative study of somatostatin-human serum albumin fusion proteins and natural somatostatin on receptor binding, internalization and activation 2014-02-27
发表时间：Peng Y1, Deng L1, Ding Y1, Chen Q1, Wu Y1, Yang M2, Wang Y3, Fu Q1. Author information

名称/货号：PLoS One. 2014 Feb 27;9(2):e89932

影响因子：

应用范围：

种属：
The α4 Nicotinic Receptor Promotes CD4+ T-Cell Proliferation and a Helper T-Cell Immune Response 2014-01-03
发表时间：Jacob C. Nordman, Pretal Muldoon, Sarah Clark, M. Imad Damaj and Nadine Kabbani

名称/货号：Molecular Pharmacology January 2014 vol. 85 no. 1 50-61

影响因子：

应用范围：

种属：
p21-Activated Kinase (PAK) Regulates Cytoskeletal Reorganization and Directional Migration in Human Neutrophils 2013-09-03
发表时间：Itakura A1, Aslan JE, Kusanto BT, Phillips KG, Porter JE, Newton PK, Nan X, Insall RH, Chernoff J, McCarty OJ.

名称/货号：PLoS One. 2013 Sep 3;8(9):e73063

影响因子：

应用范围：

种属：
The Cdc42 Guanine Nucleotide Exchange Factor FGD6 Coordinates Cell Polarity and Endosomal Membrane Recycling in Osteoclasts 2013-06-27
发表时间：Steenblock C1, Heckel T1, Czupalla C1, Espírito Santo AI1, Niehage C1, Sztacho M1, Hoflack B2.

名称/货号：J Biol Chem. 2014 Jun 27;289(26):18347-59

影响因子：

应用范围：

种属：
Vibrio parahaemolyticus Effector Proteins Suppress Inflammasome Activation by Interfering with Host Autophagy Signaling 2013-01-09
发表时间：Higa N1, Toma C, Koizumi Y, Nakasone N, Nohara T, Masumoto J, Kodama T, Iida T, Suzuki T.

名称/货号：PLoS Pathog. 2013 Jan;9(1):e1003142

影响因子：

应用范围：

种属：
Invadopodia and rolling-type motility are specific features of highly invasive p190bcr-abl leukemic cells 2012-11-03
发表时间：ThomasDaubon, TristanRochelle, NicolasBourmeyster

名称/货号：European Journal of Cell Biology Volume 91, Issues 11–12, November–December 2012, Pages 978–987

影响因子：

应用范围：

种属：
Epithelial junction formation requires confinement of Cdc42 activity by a novel SH3BP1 complex 2012-08-20
发表时间：Elbediwy A1, Zihni C, Terry SJ, Clark P, Matter K, Balda MS.

名称/货号：J Cell Biol. 2012 Aug 20;198(4):677-93

影响因子：

应用范围：

种属：
MMP-9 and CXCL8/IL-8 Are Potential Therapeutic Targets in Epidermolysis Bullosa Simplex 2012-07-19
发表时间：Lettner T1, Lang R, Klausegger A, Hainzl S, Bauer JW, Wally V.

名称/货号：PLoS One. 2013 Jul 19;8(7):e70123

影响因子：

应用范围：

种属：
Interferon gamma induces actin polymerization, Rac1 activation and down regulates phagocytosis in human monocytic cells 2012-01-03
发表时间：Frausto-Del-Río D1, Soto-Cruz I, Garay-Canales C, Ambriz X, Soldevila G, Carretero-Ortega J, Vázquez-Prado J, Ortega E.

名称/货号：Cytokine. 2012 Jan;57(1):158-68

影响因子：

应用范围：

种属：
The Aarskog-Scott Syndrome Protein Fgd1 Regulates Podosome Formation and Extracellular Matrix Remodeling in Transforming Growth Factor beta-Stimulated Aortic Endothelial Cells 2011-11-30
发表时间：Daubon T1, Buccione R, Génot E.

名称/货号：Mol Cell Biol. 2011 Nov;31(22):4430-41

影响因子：

应用范围：

种属：
Polarized migration of lymphatic endothelial cells is critically dependent on podoplanin regulation of Cdc42 2011-01-03
发表时间：Navarro A1, Perez RE, Rezaiekhaligh MH, Mabry SM, Ekekezie II.

名称/货号： Am J Physiol Lung Cell Mol Physiol. 2011 Jan;300(1):L32-42

影响因子：

应用范围：

种属：

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