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neweastbio@126.com
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| 价格 | ￥4800.00 |
| 品牌 | NewEast Biosciences  |
| 产地 | 中国 武汉 |
| 货号 | 26902 |
| 规格 | 30μl |
| 说明书 | 说明书下载 |
Active Gα13 GTP [26902]
[30 µL]
| Catalog Number: 26902 |
| Gene Symbol: Gna13 |
| Description: Anti Active Gα13 Mouse Monoclonal Antibody |
| Background: Heterotrimeric G proteins are essential cellular signal transducers. Gα13 is one of the G proteins that could mediate cell migration and angiogenesis. Other biochemical and physiological functions of Gα13 are being explored. |
| Immunogen: Recombinant full length active Gα13 protein |
| Applications: IP, IHC |
| Recommended Dilutions: 1 µg for 1~2 mg total cellular proteins for IP; 1:100 dilution for IHC |
| Concentration: 1 mg/ml |
| Host: mouse |
| Format: Liquid |
| Clonality: Monoclonal |
| Isotype: IgG2b |
| Purity: Purified from ascites |
| Storage buffer: |
| Preservative: no |
| Constituents: PBS (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 50% glycerol |
| Species Reactivity: Anti active Gα13 monoclonal antibody recognizes active Gα13 of vertebrates. |
| Storage Conditions: Store at -20°C. Avoid freeze / thaw cycles |
| Immunoprecipitation/Western blot: |
 |
IP/WB analysis of active Gα13 proteins. Purified Gα13 proteins were treated with GDP (lane 1) or GTPGTPγS (lane 2). After immunoprecipitation with anti active Gα13 GTP mouse monoclonal antibody (Cat. # 26902), these proteins were separated by SDS/PAGE and western blotted with anti Gα13 mouse monoclonal antibody (Cat. # 26004). |
| Immunohistochemistry: |
|
Staining of MEF cells (treated with PDGF) with anti Ga13 GTP monoclonal antibody showing the localization of Gα13 GTP in the leading edge of migrating cells. |
| Related Products | |||
| Catalog# | Name | Size | Price |
| 80401 | Gα13 Activation Assay Kit | 20 assays | ￥6800 |
| 26902 | Active Gα13 GTP Monoclonal Antibody | 30 μL | ￥4800 |
| 26004 | Anti Gα13 Mouse Monoclonal Antibody | 100 μL | ￥2800 |
| 21005 | Anti Gα13 Rabbit Polyclonal Antibody | 100 μL | ￥2600 |
| Publications: |
| 1. Resistance to inhibitors of cholinesterase-8A (Ric-8A) is critical for growth factor receptor-induced actin cytoskeletal reorganization J Biol Chem. 2011 Sep 2;286(35):31055-61 |
| 2. Agonist activation of the G protein-coupled receptor GPR35 involves transmembrane domain III and is transduced via Galpha and beta-arrestin-2 Br J Pharmacol. 2011 Feb;162(3):733-48 |
| 3. Gα12/13 inhibition enhances the anti-cancer effect of bortezomib through PSMB5 downregulation Carcinogenesis (2010) |
| 4. Suppression of tumor angiogenesis by Galpha(13) haploinsufficiency J Biol Chem. 2009 Oct 2;284(40):27409-15 |
发表时间：2017年
名称/货号：Gα13 Pulldown Activation Assay Kit / 80401
影响因子：Thromb Res IF=7.5
应用范围：IP-WB
种属：Human
发表时间：2021年
名称/货号：Gα13 Pulldown Activation Assay Kit /
影响因子：Sci Signal IF=7.3
应用范围：IP-WB
种属：Human
