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| 价格 | ￥4800.00 |
| 品牌 | NewEast Biosciences  |
| 产地 | 中国 武汉 |
| 货号 | 26164 |
| 规格 | 100μl |
| 说明书 | 说明书下载 |
IDH2(R172W) [26164]
[100 µL]
| Catalog Number: 26164 |
| Gene Symbol: IDH2(R172W) |
| Description: Anti IDH2(R172W) Mouse Monoclonal Antibody |
| Background: Isocitrate dehydrogenase (IDH) catalyze the oxidative decarboxylation of isocitrate to 2-oxoglutarate. The isocitrate and isopropylmalate dehydrogenases family has three members, IDH1, IDH2 and IDH3. IDH2 plays a role in intermediary metabolism and energy production. Defects in IDH2 are the cause of D-2-hydroxyglutaric aciduria type 2 (D2HGA2). Somatic mosaic mutations of this protein have also been found associated to Ollier disease and Maffucci syndrome, and R172W IDH2 mutations do exist in diffusely infiltrative gliomas. |
| Immunogen: A synthetic peptide from the internal region of IDH2 which includes the mutation of R172W, human origin. |
| Applications: ELISA, WB, IHC |
| Recommended Dilutions: ELISA: 1:1000-1:5000 WB: 1:100-1:1000 IHC: 1:50-1:100 |
| Concentration: 1 mg/ml |
| Host: Mouse |
| Format: Liquid |
| Clonality: Monoclonal |
| Isotype: |
| Purity: Purified from ascites |
| Storage buffer: |
| Preservative: no |
| Constituents: PBS (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 50% glycerol |
| Species Reactivity: recognizes IDH1(R172W) of vertebrates. |
| Storage Conditions: Store at -20°C. Avoid freeze / thaw cycles |
| Western blot: |
 |
Western blot analysis of recombinant IDH2(R172W) and wildtype proteins. Purified His-tagged IDH2(R172W) (lane 2) and wildtype protein (lane 1) were blotted with anti IDH2(R172W) monoclonal antibody (Cat. #26164). |
| Immunofluorescence: |
|
Immunofluorescence of cells expressing IDH2 proteins with anti IDH2(R172W) antibody. HEK293T cells were transfected with pCDNA3-GFP IDH2 (WT) plasmid (left column) or pCDNA3-GFP IDH2(R172W) plasmid (right column), then fixed and stained with anti-IDH2(R172W) monoclonal antibody (Cat. #26164). |
| Related Products | |||
| Catalog# | Name | Size | Price |
| 26231 | Anti IDH2(R172G) Mouse Monoclonal Antibody | 100 μL | ￥4800 |
| 26164 | Anti IDH2(R172W) Mouse Monoclonal Antibody | 100 μL | ￥4,800 |
| 26163 | Anti IDH2(R172K) Mouse Monoclonal Antibody | 100 μL | ￥4,800 |
| 26408 | Anti IDH2(R172S) Mouse Monoclonal Antibody | 100 μL | ￥4800 |
| 26165 | Anti IDH2 (R140Q) Mouse Monoclonal Antibody | 100 μL | ￥4,800 |
| 26231 | Anti IDH2(R172G) Mouse Monoclonal Antibody | 100 μL | ￥4,800 |
| 26084 | Anti IDH2(R172H) Mouse Monoclonal Antibody | 100 μL | ￥4800 |
发表时间：2020-12
名称/货号：Anti- IDH2(R172W) Mouse Monoclonal Antibody/26164
影响因子：Hum Pathol IF=2.7
应用范围：IHC(作为临床诊断的应用工具）
种属：Human
