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Anti- SMAD4(D351H) Mouse Monoclonal Antibody

价格¥4800.00
品牌NewEast Biosciences   
产地中国 武汉
货号26418
规格100μl
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  • SMAD4(D351H) [26418]
    [100]

     

    Catalog Number:  26418
    Gene Symbol:  SMAD4, DPC4, JIP, MADH4, MYHRS
    Description:   Anti SMAD4(D351H) Mouse Monoclonal Antibody
    Background:   SMAD4 family member 4, also known as SMAD4, is a protein that in human is encoded by the SMAD4 gene. It is often found mutated in many cancers. It acts as a tumor suppressor that functions in the regulation of the TGF-? signal transduction pathway, which negatively regulates growth of epithelial cells and the extracellular matrix (ECM). SMAD4 alterations have been found in multiploid colorectal cancer and pancreatic carcinoma. It is found inactivated in at least 50% of pancreatic cancers. It is also found mutated in the autosomal dominantdisease juvenile polyposis syndrome (JPS).
    Immunogen:   A synthetic peptide from the internal region of SMAD4 which includes the mutation of D351H, human origin.
    Applications:   ELISA, WB, IHC
    Recommended Dilutions:   
    ELISA: 1:1000-1:2000 
    WB: 1:500-1:1000 
    IHC: 1:50-1:100
    Concentration:   1 mg/ml
    Host:   Mouse
    Format:   Liquid
    Clonality:   Monoclonal
    Isotype:   
    Purity:   Purified from ascites
    Storage buffer:
    Preservative:  no
    Constituents:  PBS (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 50% glycerol
    Species Reactivity:  Recognizes D351H mutant, but not wild-type SMAD4 of vertebrates.
    Storage Conditions:   Store at -20°C. Avoid freeze / thaw cycles
     
    Western blot:  
    Western blot analysis of recombinant SMAD4(D351H) and wildtype proteins. Purified His-tagged SMAD4(D351H) protein (lane1) and corresponding wild-type protein (1-552aa, 65kd) (lane2) were blotted with Anti SMAD4(D351H) monoclonal antibody (Cat. #26418).
    Immunofluorescence:  
    Immunofluorescence of cells expressing SMAD4 proteins with Anti SMAD4(D351H) antibody. HEK293T cells were transfected with pCDNA3-GFP SMAD4(D351H) plasmid , pCDNA3-GFP SMAD4(WT) plasmid or pCDNA3-GFP SMAD4(D351N) plasmid, then fixed and stained with Anti SMAD4(D351H) monoclonal antibody (Cat. #26418).

     

    Related Products
    Catalog# Name Size Price
    26453 Anti SMAD4 Mouse Monoclonal Antibody 100 μL  ¥4800
    26423 Anti SMAD4(R361S) Mouse Monoclonal Antibody 100 μL  ¥4800
    26418 Anti SMAD4(D351H) Mouse Monoclonal Antibody 100 μL  ¥4800
    26422 Anti SMAD4(R361H) Mouse Monoclonal Antibody 100 μL  ¥4800

     

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