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Anti- CTNNB1(D32N) Mouse Monoclonal Antibody

价格¥4800.00
品牌NewEast Biosciences   
产地中国 武汉
货号26303
规格100μl
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  • CTNNB1(D32N) [26303]
    [100ul]

     

    Catalog Number:  26303
    Gene Symbol:  Beta-catenin, CTNNB, Catenin beta-1
    Description:   Anti CTNNB1(D32N) Mouse Monoclonal Antibody
    Background:   CTNNB1 protein is a dual function protein. It is a subunit of a complex of proteins that from adherens junctions, which are important for the establishment and maintenance of epithelial cell layers by regulating cell growth and adhesion between adjacent cells. CTNNB1 protein also pulls double duty as an intracellular signal transducer in the Wnt signaling pathway. Mutations of CTNNB1 have been implicated in the pathogenesis of several cancers.
    Immunogen:   A synthetic peptide from the internal region of CTNNB1 which includes the mutation of D32N, human origin.
    Applications:   ELISA, WB, IHC
    Recommended Dilutions:   
    ELISA: 1:1000-1:5000 
    WB: 1:500-1:1000 
    IHC: 1:50-1:100
    Concentration:   1 mg/ml
    Host:   Mouse
    Format:   Liquid
    Clonality:   Monoclonal
    Isotype:   
    Purity:   Purified from ascites
    Storage buffer:
    Preservative:  no
    Constituents:  PBS (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 50% glycerol
    Species Reactivity:  Recognizes D32N mutant, but not wild-type CTNNB1 of vertebrates.
    Storage Conditions:   Store at -20°C. Avoid freeze / thaw cycles
     
    Western blot:  
    Western blot analysis of recombinant CTNNB1(D32N) and wildtype proteins. Purified His-tagged CTNNB1(D32N) protein (lane2) and corresponding wild-type protein (lane1) were blotted with Anti CTNNB1(D32N) monoclonal antibody (Cat. #26303).
    Immunofluorescence:  
    Immunofluorescence of cells expressing CTNNB1 proteins with Anti CTNNB1(D32N) antibody. HEK293T cells were transfected with pCDNA3-GFP CTNNB1(WT) plasmid (left column) or pCDNA3-GFP CTNNB1(D32N) plasmid (right column), then fixed and stained with Anti CTNNB1(D32N) monoclonal antibody (Cat. #26303).

     

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