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Anti-BRAF(V600A) Mouse Monoclonal Antibody

价格¥4800.00
品牌NewEast Bisciences   
产地
货号26199
规格100μl
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  • BRAF(V600A) [26199]
    [100UL]

     

    Catalog Number:  26199
    Gene Symbol:  BRAF, BRAF1, RAFB1
    Description:   Anti BRAF(V600A) Mouse Monoclonal Antibody
    Background:   BRAF belongs to a family of serine-threonine protein kinases. As a part of a signaling pathway known as the the RAS/MAPK pathway, it plays an important part in many cellular processes. The processes include cell proliferation, differentiation and transcriptional regulation. Mutations in the BRAF gene cause diseases. Inherited mutations in BRAF cause cardiofaciocutaneous syndrome. Acquired mutations in BRAF have been found in cancers.
    Immunogen:   A synthetic peptide from the internal region of BRAF which includes the mutation of V600A, human origin.
    Applications:   ELISA, WB, IHC
    Recommended Dilutions:   
    ELISA: 1:1000-1:5000 
    WB: 1:500-1:1000 
    IHC: 1:50-1:100
    Concentration:   1 mg/ml
    Host:   Mouse
    Format:   Liquid
    Clonality:   Monoclonal
    Isotype:   
    Purity:   Purified from ascites
    Storage buffer:
    Preservative:  no
    Constituents:  PBS (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 50% glycerol
    Species Reactivity:  Recognizes V600A mutant, but not wild-type BRAF of vertebrates.
    Storage Conditions:   Store at -20°C. Avoid freeze / thaw cycles
     
    Western blot:  
    Western blot analysis of recombinant BRAF proteins. Purified His-tagged BRAF(V600A) protein (lane 1) , corresponding wildtype protein (lane 2) , His-tagged BRAF(V600R) protein (lane 3), His-tagged BRAF(V600M) protein (lane 4), His-tagged BRAF(V600K) protein (lane 5), His-tagged BRAF(V600G) protein (lane 6), His-tagged BRAF(V600D) protein (lane 7) and His-tagged BRAF(V600E) protein (lane 8) were blotted with Anti BRAF(V600A) monoclonal antibody (Cat. #26199).
    Immunofluorescence:  

    Immunofluorescence of cells expressing BRAF proteins with Anti BRAF(V600A) antibody. HEK293T cells were transfected with pCDNA3-GFP BRAF(WT) plasmid, pCDNA3-GFP BRAF(V600A) plasmid, pCDNA3-GFP BRAF(V600D) plasmid, pCDNA3-GFP BRAF(V600G) plasmid pCDNA3-GFP-BRA (V600M) plasmid or pCDNA3-GFP BRAF(V600E) plasmid, then fixed and stained with Anti BRAF(V600A) monoclonal antibody (Cat. #26199).

     

    Related Products
    Catalog# Name Size Price
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    26129 anti BRaf Mouse Monoclonal Antibody  100 μL  ¥2800 
    26109 anti BRaf(N581S) Mouse Monoclonal Antibody  100 μL  ¥4800
    26039 anti BRaf(V600E) Mouse Monoclonal Antibody  100 μL  ¥4800
    26110 anti BRaf(V600K) Mouse Monoclonal Antibody  100 μL  ¥4800
    26184 anti BRaf(V600D) Mouse Monoclonal Antibody  100 μL  ¥4800
    26182 anti BRaf(V600R) Mouse Monoclonal Antibody  100 μL  ¥4800
    26196 anti BRaf(G469E) Mouse Monoclonal Antibody  100 μL  ¥4800
    26199 anti BRaf(V600A) Mouse Monoclonal Antibody  100 μL  ¥4800
    26462 anti BRAF(V600M) Mouse Monoclonal Antibody  100 μL  ¥4800

     

     

    Publications:
    1.  Immunohistochemistry with a mutation-specific monoclonal antibody as a screening tool for the BRAFV600E mutational status in primary cutaneous malignant melanoma
        Mod Pathol. 2013 Mar;26(3):414-20.
    2.  Immunohistochemistry as a quick screening method for clinical detection of BRAF(V600E) mutation in melanoma patients
        Tumor Biology. June 2014, Volume 35, Issue 6, pp 5727-5733
    3.  Prospective immunohistochemical analysis of BRAF V600E mutation in melanoma
        human pathology. February 2015Volume 46, Issue 2, Pages 169–175
    4.  Comparison of 2 monoclonal antibodies for immunohistochemical detection of BRAF V600E mutation in malignant melanoma, pulmonary carcinoma, gastrointestinal carcinoma, thyroid carcinoma, and gliomas
        human pathology. November 2013Volume 44, Issue 11, Pages 2563–2570
    5.  BRAF V600E Mutation-Specific Antibody: A Review
        Seminars in Diagnostic Pathology

     

     

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