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Anti- KIT(D820E) Mouse Monoclonal Antibody

价格¥4800.00
品牌NewEast Biosciences   
产地中国 武汉
货号26274
规格100μl
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  • KIT(D820E) [26274]
    [100ul]

     

    Catalog Number:  26274
    Gene Symbol:  C-Kit; CD117; KIT; PBT; SCFR
    Description:   Anti KIT(D820E) Mouse Monoclonal Antibody
    Background:   Tyrosine-protein kinase Kit, also known as proto-oncogene c-Kit or CD117 or Mast/stem cell growth factor receptor (SCFR), is a receptor tyrosine kinase protein that in humans is encoded by the KIT gene. Activating mutations in this gene are associated with gastrointestinal stromal tumors, testicular seminoma, mast cell disease, melanoma, acute myeloid leukemia, while inactivating mutations are associated with the genetic defect piebaldism.
    Immunogen:   A synthetic peptide from the internal region of KIT which includes the mutation of D820E, human origin.
    Applications:   ELISA, WB, IHC
    Recommended Dilutions:   
    ELISA: 1:1000-1:5000 
    WB: 1:500-1:1000 
    IHC: 1:50-1:100
    Concentration:   1 mg/ml
    Host:   Mouse
    Format:   Liquid
    Clonality:   Monoclonal
    Isotype:   
    Purity:   Purified from ascites
    Storage buffer:
    Preservative:  no
    Constituents:  PBS (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 50% glycerol
    Species Reactivity:  Recognizes D820E mutant, but not wild-type KIT of vertebrates.
    Storage Conditions:   Store at -20°C. Avoid freeze / thaw cycles
     
    Western blot:  
    Western blot analysis of recombinant KIT(D820E) and wild-type proteins. Purified His-tagged KIT(D820E) protein (lane 1) and corresponding wild-type protein (lane 2) were blotted with Anti KIT(D820E) monoclonal antibody (Cat. #26274).
    Immunofluorescence:  
    Immunofluorescence of cells expressing KIT proteins with Anti KIT(D820E) antibody. HEK293T cells were transfected with pCDNA3-GFP KIT(WT) plasmid (left column) or pCDNA3-GFP KIT(D820E) plasmid (right column), then fixed and stained with anti KIT(D820E) monoclonal antibody (Cat. #26274).

     

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