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neweastbio@126.com
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| 价格 | ￥4800.00 |
| 品牌 | NewEast Biosciences  |
| 产地 | 中国 武汉 |
| 货号 | 21202 |
| 规格 | 100ul |
| 说明书 | 说明书下载 |
ERBB2 [21202]
[100 µL]
| Catalog Number: 21202 |
| Gene Symbol: ERBB2/HER2/Neu |
| Description: Anti ErbB2 Rabbit Polyclonal Antibody |
| Background: The ErbB family is composed of four plasma membrane-bound receptor tyrosine kinases. Amplification or over-expression of the ERBB2 gene occurs in approximately 30% of breast cancers. HER2 is encoded by ERBB2, a known proto-oncogene located at the long arm of human chromosome 17(17q21-q22). HER2 proteins have been shown to form clusters in cell membranes that may play a role in tumorigenesis. |
| Immunogen: A synthetic peptide from the central of ErbB2 protein. |
| Applications: ELISA, WB, IHC |
| Recommended Dilutions: ELISA: 1:500-1:5000 WB: 1:200-1:2000 IHC: 1:20-1:200 |
| Concentration: 0.2 mg/ml |
| Host: Rabbit |
| Format: Liquid |
| Clonality: Polyclonal |
| Isotype: |
| Purity: Purified from serum |
| Storage buffer: |
| Preservative: no |
| Constituents: PBS (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 50% glycerol |
| Species Reactivity: Recognizes ErbB2 of vertebrates. |
| Storage Conditions: Store at -20°C. Avoid freeze / thaw cycles. |
| Western blot: |
 |
Western blot analysis of ErbB2 protein. Purified full length His-tagged ErbB2 protein (Cat. #10208) was blotted with Anti ErbB2 rabbit polyclonal antibody (Cat. #21202). |
|
Immunohistochemical analysis of paraffin-embedded human breast cancer tissue with Anti ErbB2 polyclonal antibody (Cat. #21202). Tissue samples were fixed with formaldehyde and blocked with 1% serum for 15 min at 37 °C. Antigen retrieval was by heat mediation in citrate buffer (pH6). Samples were then incubated with primary antibody (1:50 dilution) overnight at 4 °C. A HRP-conjugated Goat anti-Rabbit IgG (1:50) was used as secondary antibody. |
| Related Products | |||
| Catalog# | Name | Size | Price |
| 21200 | Anti ERBB2 Rabbit polyclonal Antibody | 100 μL | ￥2600 |
| 21202 | Anti ERBB2 Rabbit Polyclonal Antibody | 100 μL | ￥2600 |
| 26080 | Anti ERBB2(V777A) mouse monoclonal Antibody | 100 μL | ￥4800 |
| 26127 | Anti ERBB2 mouse monoclonal Antibody | 100 μL | ￥2800 |
