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| 价格 | ￥4800.00 |
| 品牌 | NewEast Biosciences  |
| 产地 | 中国 武汉 |
| 货号 | 26912 |
| 规格 | 30μl |
| 说明书 | 说明书下载 |
Active Rap1-GTP [26912]
[30 µL]
| Catalog Number: 26912 |
| Gene Symbol: RAP1 |
| Description: Anti Active Rap1 Mouse Monoclonal Antibody |
| Background: Small GTPases are a super-family of cellular signaling regulators. Rap family members (Rap1A, Rap1B, Rap2A, Rap2B, and Rap2C) are Ras-like GTPases that regulate cell proliferation, differentiation, apoptosis and adhesion mechanisms. Rap1 (Ras-proximate-1) regulates apoptosis through interaction with two MAP3K effectors of the MEK/ERK MAP-kinase pathway, Raf-1 and B-Raf. Rap1 regulates cell adhesion mechanisms through effector molecules such as the integrins and cadherins. |
| Immunogen: Recombinant full length protein of active Rap1. |
| Applications: IP, IHC |
| Recommended Dilutions: 1 μg for 1~2 mg total cellular proteins for IP 1:100 dilution for IHC |
| Concentration: 1 mg/ml |
| Host: mouse |
| Format: Liquid |
| Clonality: Monoclonal |
| Isotype: IgG |
| Purity: Purified from ascites |
| Storage buffer: |
| Preservative: no |
| Constituents: PBS (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 50% glycerol |
| Species Reactivity: Anti-active Rap1 antibody recognizes active Rap1 of vertebrates. |
| Storage Conditions: Store at -20°C. Avoid freeze / thaw cycles |
| Immunoprecipitation/Western blot: |
 |
Rap1 activation assay. Purified Rap1 proteins were immunoprecipitated after treated with GDP (lane 1) or GTPγS (lane 2). Immunoprecipitation was done with the anti-active Rap1 monoclonal antibody (Cat. No. 26912). Immunoblot was with an anti-Rap1 polyclonal antibody (Cat. No. 21104). |
| Related Products | |||
| Catalog# | Name | Size | Price |
| 26912 | Active Rap1 GTP Monoclonal Antibody | 30 μL | ￥4800 |
| 81401 | Rap1 Activation Assay Kit | 20 assays | ￥6800 |
| Publications: |
| 1. Rap1 GTPase Activation and Barrier Enhancement in RPE Inhibits Choroidal Neovascularization In Vivo PLoS One. 2013 Sep 10;8(9):e73070 |
发表时间：2013-06-04
名称/货号：Anti- Active Rap1 Mouse Monoclonal Antibody/26912
影响因子：Scientific Reports IF=4.6
应用范围：ICC
种属：hippocampi
发表时间：2013-06-04
名称/货号：Anti- Active Rap1 Mouse Monoclonal Antibody/26912
影响因子：The Journal of Physiology IF=5.5
应用范围：IHC
种属：Mouse
发表时间：2021-06-24
名称/货号：Anti- Active Rap1 Mouse Monoclonal Antibody/26912
影响因子：The FASEB Journal IF=4.8
应用范围：IHC
种属：Human
发表时间：2023-03-09
名称/货号：Rap1 Pulldown Activation Assay Kit/81401
影响因子：Cell & Bioscience IF=7.5
应用范围：IP-WB
种属：Human
发表时间：2024-03-14
名称/货号：Rap1 Pulldown Activation Assay Kit/81401
影响因子：Advanced Science IF=15.1
应用范围：IP-WB
种属：Mouse
