100μL

Product Name: Rab7 Protein

Synonyms: Member RAS oncogene family, Rab7a, MGC102153

Catalogue Number:  10167

Source: Human, recombinant full length, His₆-tag

Expression Host: E. coli

Molecular Weight: 23 kDa

Purity: >95% by SDS-PAGE

Introduction: Members of the Rab family of Ras-related GTP-binding proteins are important regulators of vesicular transport and are located in specific intracellular compartments. Rab7 has been localized to late endosomes and shown to be important in the late endocytic pathway. In addition, it has been shown to have a fundamental role in the cellular vacuolation induced by the cytotoxin VacA of Helicobacter pylori.

Amino Acid Sequence (1-207)

MTSRKKVLLKVIILGDSGVGKTSLMNQYVNKKFSNQYKATIGADFLTKEVMVDDRLVTMQIWDTAGQERFQSLGVAFYRGADCCVLVFDVTAPNTFKTLDSWRDEFLIQASPRDPENFPFVVLGNKIDLENRQVATKRAQAWCYSKNNIPYFETSAKEAINVEQAFQTIARNALKQETEVELYNEFPEPIKLDKNDRAKASAESCSC

Properties

Physical Appearance (form):  Lyophilized Powder from 20 mM Tris-HCl, pH 7.4, containing 100 mM NaCl.

Physical Appearance (color): White

Solubility (Color): colorless

Solubility (Turbidity): clear, 10 mg/mL H₂O

Storage:  -80°C

Preparation Instructions

Centrifuge the vial before open the cap and reconstitute in water. Adding of 10 mM b-mercaptoethanol or 1 mM DTT into the solution to protect the protein is recommended and using of non-ionic detergents such as n-Dodecyl b-D-maltoside (DoDM) or polyethylene detergents (e.g. C₁₂E₁₀) also help to stabilize the protein. Avoid repeated freezing and thawing after reconstitution.

References

1.         Edinger, A. L. et al., Dev. Cell 5: 571-582, 2003.

2.         Houlden, H. et al., Ann. Neurol. 56: 586-590, 2004.

3.         Meggouh, F. et al., Neurology 67: 1476-1478, 2006.

4.         Rak, A. et al., Cell 117: 749-760, 2004.

Verhoeven, K. et al., Am. J. Hum. Genet. 72: 722-727, 2003.