100μL

Product Name: Fascin1 Protein

Synonyms: Fascin homolog 1, actin-bundling protein, FSCN1, SNL, p55

Catalogue Number:  10150

Source:  Human, recombinant full length, his-tag

Expression Host: E. coli

Molecular Weight: 55 kDa

Purity: >95% by SDS-PAGE

Introduction: Fascin is an ~58 kDa monomeric actin filament bundling protein. It is required to maximally cross-link the actin filaments into straight, compact, and rigid bundles, and contributes to the formation of filopodia that are critical for cell migration. Elevated levels of fascin have been found in metastatic tumors and are correlated with clinically aggressive phenotypes, poor prognosis, and shorter survival.

Amino Acid Sequence (1-493)

MTANGTAEAVQIQFGLINCGNKYLTAEAFGFKVNASASSLKKKQIWTLEQPPDEAGSAAVCLRSHLGRYLAADKDGNVTCEREVPGPDCRFLIVAHDDGRWSLQSEAHRRYFGGTEDRLSCFAQTVSPAEKWSVHIAMHPQVNIYSVTRKRYAHLSARPADEIAVDRDVPWGVDSLITLAFQDQRYSVQTADHRFLRHDGRLVARPEPATGYTLEFRSGKVAFRDCEGRYLAPSGPSGTLKAGKATKVGKDELFALEQSCAQVVLQAANERNVSTRQGMDLSANQDEETDQETFQLEIDRDTKKCAFRTHTGKYWTLTATGGVQSTASSKNASCYFDIEWRDRRITLRASNGKFVTSKKNGQLAASVETAGDSELFLMKLINRPIIVFRGEHGFIGCRKVTGTLDANRSSYDVFQLEFNDGAYNIKDSTGKYWTVGSDSAVTSSGDTPVDFFFEFCDYNKVAIKVGGRYLKGDHAGVLKASAETVDPASLWEY

Properties

Physical Appearance (form):  Lyophilized Powder from 20 mM Tris-HCl, pH 7.4, containing 100 mM

NaCl.

Physical Appearance (color): White

Solubility (Color): colorless

Solubility (Turbidity): clear, 10 mg/mL H₂O

Storage:  -80°C

Preparation Instructions

Centrifuge the vial before open the cap and reconstitute in water. Adding of 10 mM b-mercaptoethanol or 1 mM DTT into the solution to protect the protein is recommended and using of non-ionic detergents such as n-Dodecyl b-D-maltoside(DoDM) or polyethylene detergents (e.g. C₁₂E₁₀) also help to stabilize the protein. Avoid repeated freezing and thawing after reconstitution.

References

1.        Mosialos, G. et al., Am. J. Path. 148: 593-600, 1996.

2.        Ono, S. et al., J. Biol. Chem. 272: 2527-2533, 1997.

3.        Pinkus, G. S. et al., Am. J. Path. 150: 543-562, 1997.

4.        Sonderbye, L. et al., Adv. Exp. Med. Biol. 471: 41-46, 1997.

5.        Yamakita, Y. et al., J. Biol. Chem. 271: 12632-12638, 1996.

6.        Yamashiro-Matsumura, S. et al., J. Biol. Chem. 260: 5087-5097, 1985.

Zhang, J. et al., Science 325: 1250-1254, 2009.