100μL

Product Name: RhoA Protein
Synonyms: Ras homolog gene family, member A, ARHA, ARH12, RHO12, RHOH12
Catalogue Number:  10104
Source: Human, recombinant full length, His6-tag
Expression Host: E. coli
Molecular Weight:  22 kDa
Purity:  >95% by SDS-PAGE

Introduction:  Small GTPases are a super-family of cellular signaling regulators. RhoA belongs to the Rho sub-family of GTPases. Rho proteins play critical roles in many actin cytoskeleton- dependent processes including platelet aggregation, cell motility, contraction, and cytokinesis. It regulates the formation of stress fibers and focal adhesions in fibroblasts and Ca2+ sensitivity of smooth muscle contraction.

Amino Acid Sequence (1-193)
MAAIRKKLVIVGDGACGKTCLLIVFSKDQFPEVYVPTVFENYVADIEVDGKQVELALWDTAGQEDYDRLRPLSYPDTDVILMCFSIDSPDSLENIPEKWTPEVKHFCPNVPIILVGNKKDLRNDEHTRRELAKMKQEPVKPEEGRDMANRIGAFGYMECSAKTKDGVREVFEMATRAALQARRGKKKSGCLVL

Properties
Physical Appearance (form):  Lyophilized Powder from 20 mM Tris-HCl, pH 7.4, containing 100 mM NaCl.
Physical Appearance (color): White
Solubility (Color): colorless
Solubility (Turbidity): clear, 10 mg/mL H2O

Storage:  -80°C

Preparation Instructions
Centrifuge the vial before open the cap and reconstitute in water. Adding of 10 mM -mercaptoethanol or 1 mM DTT into the solution to protect the protein is recommended and using of non-ionic detergents such as n-Dodecyl -D-maltoside (DoDM) or polyethylene detergents (e.g. C12E10) also help to stabilize the protein. Avoid repeated freezing and thawing after reconstitution.

References
1. Canman, J. C. et al., Science 322: 1543-1546, 2008.
2. Guilluy, C. et al., Nature Med. 16: 183-190, 2010.
3. Machacek, M. et al., Nature 461: 99-103, 2009
4. Nakamura, M. et al., Invest. Ophthal. Vis. Sci. 42: 941-947, 2001.
5. Rao, P. V. et al., Invest. Ophthal. Vis. Sci. 42: 1029-1037, 2001.
6. Valderrama, F. et al., Science 311: 377-381, 2006.
7. Wang, H.-R. et al., Science 302: 1775-1779, 2003.
8. Wu, K. Y. et al., Nature 436: 1020-1024, 2005.
9. Yoshida, S.et al., Science 313: 108-111, 2006.