30 μL

Active Cdc42
Catalog Number: 26905
Gene Symbol: CDC42
Description: Anti-Active Cdc42 Mouse Monoclonal Antibody

Background: Small GTPases are a super-family
of cellular signaling regulators. Cdc42 belongs
to the Rho sub-family of GTPases that regulate
cell motility, cell division, and gene transcription.
GTP binding increases the activity of Cdc42, and
the hydrolysis of GTP to GDP renders it inactive.
GTP hydrolysis is aided by GTPase activating
proteins (GAPs), while exchange of GDP for GTP
is facilitated by guanine nucleotide exchange
factors (GEFs).
Immunogen: Recombinant full length protein of
active Cdc42
Tested applications: IP, IHC
Recommended dilutions:
1 μg for 1~2 mg total cellular proteins
Concentration: 1 mg/ml
Host: mouse
Clonality: Monoclonal
Isotype: IgG
Purity: Purified from ascites
Format: Liquid
Storage buffer:
Preservative: no
Constituents: PBS (without Mg2+ and Ca2+), pH
7.4, 150 mM NaCl, 50% glycerol
Species Reactivity: Anti-active Cdc42 antibody
recognizes active Cdc42 from vertebrates.
Storage Conditions: Store at -20°C. Avoid
freeze / thaw cycles

Immunoprecipitation and Western blot:

 

IP/WB analysis of active Cdc42 protein. Purified
recombinant Cdc42 proteins were loaded with
GDP (lane 1) or GTPgS (lane 2). These proteins
were immunoprecipitated with anti-active Cdc42
mouse monoclonal antibody (Cat. # 26905).
After SDS/PAGE, the membrane filter was
probed with anti-Cdc42 mouse monoclonal
antibody (Cat. # 26008).