Active Rac1
Catalog Number: 26903
Gene Symbol: RAC1
Description: Anti-Active Rac Mouse Monoclonal Antibody

Background: Small GTPases are a super-family
of cellular signaling regulators. Rac belongs to
the Rho sub-family of GTPases that regulate cell
motility, cell division, and gene transcription.
GTP binding increases the activity of Rac, and the
hydrolysis of GTP to GDP renders it inactive.
GTP hydrolysis is aided by GTPase activating
proteins (GAPs), while exchange of GDP for GTP
is facilitated by guanine nucleotide exchange
factors (GEFs).
Immunogen: Recombinant full length protein of
active Rac1
Tested applications: IP, IHC
Recommended dilutions:
1 μg for 1~2 mg total cellular proteins
Concentration: 1 mg/ml
Host: mouse
Clonality: Monoclonal
Isotype: IgG
Purity: Purified from ascites
Format: Liquid
Storage buffer:
Preservative: no
Constituents: PBS (without Mg2+ and Ca2+), pH
7.4, 150 mM NaCl, 50% glycerol
Species Reactivity: Anti-active Rac1 antibody
recognizes active Rac1 from vertebrates.
Storage Conditions: Store at -20°C. Avoid
freeze / thaw cycles

Immunoprecipitation and Western blot:

 

Rac activation assay. MEF cells were treated
with (lane 2) or without (lane 1) PDGF. Cell
lysates were incubated with an anti-active Rac
monoclonal antibody (Cat # 26903) (top panel).
The precipitated active Rac was immunoblotted
with an anti-Rac rabbit polyclonal antibody (Cat
# 21003). The bottom panel shows the Western
blot with anti-Rac of the cell lysates used (5% of
that used in the top panel).